Technical Resources
DyLight® Fluorescent Conjugates
Columbia Biosciences recently introduced DyLight® Fluors conjugated to Streptavidin and Anti-epitope tag antibodies to be used for High Throughput Screening and Fluorescent Microscopy.
DyLight® Fluors have absorption maxima ranging from 350nm to 777nm, covering the entire visible light spectrum and several key near-infared and infrared wavelengths. Both the absorption and emission properties of the DyLight® Fluors match the output (excitation) and detection wavelengths of common fluorescence instrumentation. The chart below details the specral properties of the DyLight® Fluorescent Dyes.
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Literature
Cyanobacterial stabilized phycobilisomes as fluorochromes for extracellular antigen detection by flow cytometry
Telford - J. Immun. Methods, 2001
PBXL Fluorescent Dyes for Ultrasensitive Direct Detection
Zoha - J. Fluorescence, 1999
MicroPlate Detection comparison between SureLight®
P-3L, other fluorophores and enzymatic detection
P-3L, other fluorophores and enzymatic detectionColumbia Biosciences, 2010
Introduction to TR-FRET Assays
Columbia Biosciences, 2010
