Applications
Quasar® 570WS (Q570WS) is useful in fluorescence microscopy and flow cytometry as well as high-throughput screening applications in both TR-FRET and Fluorescence Polarization (FP) formats. It can easily be substituted for phycoeryrthin (PE), Cy™3, Alexa Fluor® 546, and other similar dyes.
Characteristics
The unique structure modifications of Q570WS make this dye more more water soluble than other organic small molecule dyes, such as Cy™3. This increased water solubility results in conjugates that produce less background than other organic dye conjugates and have a longer shelf-life. The molecular weight of Q570WS is 727.68 Daltons.
Spectral Characteristics
| Absorption maximum | 555 nm |
| Additional Absorption peak | 515 nm |
| Emission maximum | 571 nm |
| Extinction Coefficient at 565 nm | 1.33 x 105 M-1cm-1 |
| Extinction Coefficient at 280 nm | 1.65 x 105 M-1cm-1 |
Specifications
| A550/A280 ≥ 8.0 |
‘Black Hole Quencher,’ and ‘Quasar’ are trademarks of and licensed by Biosearch Technologies, Inc., Novato, CA. The BHQ, CAL Fluor, and Quasar dye technology is the subject of existing or pending patents including US Patent No. 7,019,129 and is licensed and sold under agreement with Biosearch Technologies, Inc. Products incorporating this technology are to be used for research and development only and their use in any in vitro diagnostic application is strictly prohibited without the express written permission of Biosearch Technologies, Inc.