Applications for B-Phycoerythrin
Because of its high quantum yield, B-PE is considered the world’s brightest fluorophore. It is compatible with commonly available lasers and gives exceptional results in flow cytometry, Luminex® and immunofluorescent staining. B-PE is also less “sticky” than common synthetic fluorophores and therefore gives less background interference.
Structural Characteristics
B-phycoerythrin is produced by red algae such as Rhodella sp.. The protein is made up of at least three different subunits and varies according to the species of algae that produces it. The subunit structure of the most common B-PE is (αβ)6γ. The α subunit has two phycoerythrobilins (PEB), the β subunit has 3 PEBs and the γ subunit has 2 PEB and 2 phycourobilins (PUB). The molecular weight of B-PE is 240,000 Daltons.
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Excitation and emission profiles for SureLight® B-PE. (Emission scan excitation wavelength at 540 nm.) Compatible with:
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Absorbamce spectrum of SureLight® R-PE in 100 mM sodium phosphate (pH 7.4) + 100 mM NaCl + 2 mM sodium azide. |
Spectral Characteristics
| Absorption maximum | 545 nm |
| Additional Absorption peak | 563.5 |
| Emission maximum | 572 nm |
| Extinction Coefficient (ε) | 2.41 x 106 M-1cm-1 |
| Quantum Yield (QY) | 0.98 |
| Brightness (ε x QY) | 2.36 x 106 M-1cm-1 |
Specifications
| A545/A280 ≥ 5.5 |
| A620/A545 < 0.01 |
| >98% single peak by HPLC |