Applications for Peridinin Chlorophyll Protein Complex
Peridinin Chlorophyll (PerCP) is commonly used in immunoassays such as FACS and flow cytometry.
Structural Characteristics
Peridinin is a light-harvesting pigment associated with chlorophyll and found in the peridinin chlorophyll binding protein (PerCP); a light harvesting protein found in dinoflagellates. The PerCP complex has a high ratio of peridinin to chlorophyll; most light-harvesting complexes contain more chlorophyll than carotenoids, but the PerCP contains eight peridinin and two chlorophyll molecules arranged to promote peridinin-chlorophyll energy transfer. The PerCP complex is a trimer with a molecular weight of 35kDa.
| Spectral Characteristics | Specifications |
| Absorption maximum | 483 nm | A483/A280 ≥ 4.6 | |
| Emission maximum | 676 nm | Absorbance max. 483 ± 1 nm | |
| Extinction Coefficient (ε) | 1.96 x 106 M-1cm-1 | Emission max. 676 ± 1 nm |
| >95% single peak by HPLC |
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